EDUCATION

- Ph.D. Microbial Ecology (2000), University of Lyon
- M.S. Microbial Ecology (1996), University of Lyon
- B.S. Biochemistry (1995)
- B.T.S. Biology and Biochemistry (1993), ESTBB Lyon

RESEARCH INTERESTS

My research interests focused many facets of microbial ecology. During my PhD, I studied the involvement of horizontal gene transfers (HGT) in the adaptation and evolution of bacteria to their environments with a particular look to the transforming effect of lightning-mediated electrical conditions. During the last few years, I’ve also investigated the genetic potential of environmental bacteria with metagenomic approaches. And more recently, I went into the study of horizontal gene transfer between transgenic plants and soil bacteria.

PUBLICATIONS

S. Demanèche, J-P.Chapel, L. Jocteur Monrozier, and H. Quiquampoix (2009) Dissimilar pH-dependent adsorption features of bovine serum albumin and a-chymotrypsin on mica probed by AFM. Colloids and Surfaces B: Biointerfaces, in press, DOI: 10.1016/j.colsurfb.2008.12.036.

S. Demanèche, L. Philippot, M. M. David, E. Navarro, T.M. Vogel and P. Simonet (2009) Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach. Applied and Environmental Microbiology, vol. 75, n° 2, p. 534-537.

S. Demanèche, M.M. David, E. Navarro, A. Faugier, J. Züfle, A. Saptoka, P. Simonet, and T. M. Vogel (2009) Functional Gene Enrichment from Hybridized Clones from Metagenomic Clone Libraries. Journal of Microbiological Methods, vol. 76, p. 105-107.

A. Ginolhac, S. Demanèche, C. Jarrin, P. Robe, F. Lefèvre, R. Nalin, G. Perrrière, T.M. Vogel et P. Simonet (2008) Analyse et exploitation de la diversité génétique des polykétides synthases de type I dans l’ADN métagénomique d’un sol. Les actes du BRG, vol. 7, p. 201-213.

S. Demanèche, H. Sanguin, J. Poté, E. Navarro, D. Bernillon, P. Mavingui, W. Wildi, T.M. Vogel and P. Simonet (2008) Antibiotic resistance soil bacteria in transgenic plant fields. Proceedings of the National Academy of Science, vol. 105, n° 10, p.3957-3962.

S. Demanèche, H. Cérémonie, F. Buret, P. Auriol, T.M. Vogel and P. Simonet (2005) Gene transfer in soil induced by lightning . XIVth International Symposium On High Voltage Engineering, 25-29 august 2005, Beijin (China) proceeding B07.

S. Demanèche, C. Meyer, J. Micoud, M. Louwagie , J. C. Willison and Y. Jouanneau (2004) Identification and functional analysis of two aromatic ring-hydroxylating dioxygenases from a Sphingomonas strain degrading various polycyclic aromatic hydrocarbons. Applied and Environmental Microbiology, vol. 70, n° 11, p. 6714-6725.

S. Demanèche, L. Jocteur-Monrozier, J-P.Chapel and P. Simonet (2002) Influence of plasmid conformation and inserted sequence homology on natural transformation of Acinetobacter sp. Annals of Microbiology, vol. 52, n° 1, p. 61-69.

S. Demanèche, F. Bertolla, F. Buret, R. Nalin, A. Sailland, P. Auriol, T.M. Vogel and P. Simonet (2001) Laboratory-scale evidence for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, vol. 67, n° 8, p. 3440-3444.

S. Demanèche, E. Kay, F. Gourbière and P. Simonet (2001) Natural transformation of Pseudomonas fluorescens and Agrobacterium tumefaciens in soil. Applied and Environmental Microbiology, vol. 67, n° 6, p. 2617-2621.

S. Demanèche, L. Jocteur-Monrozier, H. Quiquampoix and P. Simonet (2001) Evaluation of biological and physical protection against nuclease degradation of clay-bound plasmid DNA. Applied and Environmental Microbiology, vol. 67, n° 1, p. 293-299.


PATENT

R. Nalin, S. Demanèche, F. Bertolla, F. Buret, P. Auriol, T. Vogel and P. Simonet (2001) Methods of creating genetic diversity. PCT/EP 02/07946 (EP 01 401944.2 du 19/07/2001).


POSTERS

S. Demanèche, E. Navarro, M. David, L. Philippot, R. Nalin, A. Faugier, J. Züfle, P. Simonet, T.M. Vogel (2008) Metagenomic library selection and validation by pyrosequencing. 12th International Symposium on Microbial Ecology, Cairns, Australia.

S. Demanèche, H. Sanguin, J. Poté, E. Navarro, D. Bernillon, P. Mavingui, W. Wildi, T.M. Vogel and P. Simonet (2007) Résistances bactériennes aux antibiotiques dans les sols. Colloque d’écologie microbienne, La Grande Motte, France.

S. Demanèche, J. Poté, H. Sanguin, D. Bernillon, P. Mavingui, W. Wildi, T.M. Vogel and P. Simonet (2007) Antibiotic Resistance in Soil Bacteria related to Transgenic Plants Impact: a Field Study. 9th symposium on bacterial genetics and ecology (BAGECO-9), Wernigerode, Allemagne.

H. Quiquampoix , J. Abadie, M.H. Baron, J.P. Chapel, P Déjardin, S. Demanèche, J. Grosclaude, C. Le Guernevé, S. Noinville, D. Rapp, M. Revault, H. Rezaei, P. Rigou, S. Staunton, and E.N. Vasina (2004) Mechanisms of protein adsorption and fate of prions in soil. ISMOM ("interactions of Soil Minerals with Organic Components and Microorganisms"). Wuhan, Chine. Congrès.

S. Demanèche, J. Willison and Y. Jouanneau (2003) Identification of multiple ring-hydroxylating dioxygenase genes in a chrysene-degrading  bacterial strain. 1st FEMS congress of European Microbiologists, Ljubljana, Slovénie.

H. Quiquampoix, S. Demanèche, J.P. Chapel, S. Servagent-Noinville and M.H. Baron (2003) Mechanisms of interaction of hard and soft proteins with mineral surfaces. 16th International Symposium on Environmental Biogeochemistry, Oirase, Japon.

S. Demanèche, J. Willison and Y. Jouanneau (2002) Identification of the genes responsible for PAH degradation in a chrysene-degrading  bacterial strain. Xth International Congress of Bacteriology and Applied Microbiology, Paris, France.

S. Demanèche, J. Willison and Y. Jouanneau (2002) Degradation of chrysene by Sphingomonas sp. CHY-1 : identification of catabolic specific enzymes. Microbiology of hydrocarbons: state of the art and perspectives, Rueil-Malmaison, France. Colloque.

S. Troussieux, L. Jocteur-Monrozier and P. Simonet (1999) Protection role of illite and behaviour of genes located on two different plasmids for transformation of Acinetobacter calcoaceticus. 6th symposium on bacterial genetics and ecology (BAGECO-6), Florence, Italie.


ORAL COMMUNICATIONS

S. Demanèche and P. Simonet (2008) Horizontal gene transfer between transgenic plants and soil bacteria. 10th International Symposium on the Biosafety of Genetically Modified Organisms, Wellington, New Zealand.

S. Demanèche, H. Sanguin, J. Poté, E. Navarro, D. Bernillon, P. Mavingui, W. Wildi, T.M. Vogel and P. Simonet (2007) Antibiotic resistance soil bacteria in transgenic plant fields. 2nd symposium on antimicrobial resistance in animals and the environment (ARAE 2007), Tours, France.

S. Troussieux, L. Jocteur-Monrozier and P. Simonet (1999) Genes located on two different replicons do not exhibit a similar behaviour for transformation of Acinetobacter calcoaceticus in microcosm conditions mimicking soil environments. First symposium of the EU-concerted action on "Mobile elements" contribution to bacterial adaptability and diversity (MECBAD), Le Mont Ste Odile, France.

CURRICULUM VITAE (Download my complete CV )