Horizontal Gene Transfer

The general objectives were to determine the involvement of horizontal gene transfers (HGT) in the adaptation and evolution of bacteria to new environments. Our studies focused mainly on natural genetic transformation and also, but at a lesser extent, on conjugation.

Publications

Demanèche S, Sanguin H, Poté J, Navarro E, Bernillon D, Mavingui P,  Wildi W, Vogel TM and Simonet P. 2008 Antibiotic resistant soil  bacteria in transgenic plant fields. (Proc Natl Acad. Sci. USA) PNAS. 105:3957-3962.

Mercier, A., E. Kay and P. Simonet. 2006. Horizontal gene transfer by natural transformation in soil environment. In Nannipieri, P.and K. Smalla (ed.), Nucleic Acids and Proteins in Soils. Spinger-Verlag, Berlin, Heidelberg.

Cérémonie, H.,Buret, F., Simonet, P., Vogel, T.M. 2006. Natural electrotransformation of lightning-competent Pseudomonas sp. strain N3 in artificial soil microcosms. Appl Environ Microbiol 72: 2385-2389

Ginolhac A., Jarrin C., Robe P., Perrière G., Vogel T. M. , Simonet P. and Nalin R. 2005. Type I PKS may have evolved through horizontal gene transfer. Journal of Molecular Evolution.  60:5.

Kay, E., Chabrillat, G., Vogel, T.M., Simonet, P. 2003. Intergeneric transfer of chromosomal and conjugative plasmid genes between Ralstonia solanacearum and Acinetobacter sp. BD413.  Molecular Plant-Microbe Interactions, 16:74-82.

Kay E., Bertolla F. Vogel T.M and Simonet P. 2002. Opportunistic colonization of Ralstonia solanacearum-infected plants by Acinetobacter sp. and its natural competence development. Microbiol.Ecol.43:291-297.

Demanèche S., Bertolla F., Buret F., Nalin R., Sailland A., Auriol P., Vogel T.M. and Simonet P. 2001. Laboratory-scale evidence for lightning-mediated gene transfer in soil. Appl. Environ. Microbiol. 67: 3440-3444.

Demaneche S., Kay E., Gourbiere F. and Simonet P. 2001. Natural Transformation of Pseudomonas fluorescens and Agrobacterium tumefaciens in Soil. Appl. Environ. Microbiol.;67 :2617-2621

Clerc-Bardin S., Karray F., Frostegard A., Pernodet J.-L. and Simonet P. 2001. Development of a conditional lethal system for a Streptomyces lividans strain and its use to investigate conjugative transfer in soil. FEMS Microbiol. Ecol. 38 : 115-121

Clerc S and Simonet P. 1998. A review of available systems to investigate transfer of DNA to indigenous soil bacteria. Anto. van Leew. 73:15-23.